Accelerating Pine Genomics Mississippi Genome Exploration Laboratory

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CotQuest | Sequence Read Classification Pipeline (SRCP)

CotQuest - Version 1.0

Reference: Bunge JA, Chouvarine P, Peterson DG (2009) CotQuest: Improved algorithm and software for nonlinear regression analysis of DNA reassociation kinetics data.  Analytical Biochemistry, in press.

Description: CotQuest is specifically tailored to conduct nonlinear regression analysis of DNA reassociation kinetics (i.e., Cot) data when used with the statistics package SAS® (  CotQuest is unique in that it does not require users to input guesses as to the final values of parameters.  The CotQuest program surpasses all existing Cot analysis programs with regard to automation, statistical robustness, user-friendliness, and graphical output.  CotQuest is available in two variations, CotQuestG and CotQuestU.

User Guide:
CotQuest User's Guide (pdf)

Download Program:

CotQuestG, v1.0 - For use with Windows XP or Windows Vista.  CotQuestG is packaged with a graphical user interface (GUI) that helps lead users through the Cot analysis process. 
CotQuestU, v1.0 - For use on all operating systems that are compatible with SAS® (i.e., Windows, Unix, Linux, and Macintosh).  CotQuestU does not have a GUI.

Sequence Read Classification Pipeline (SRCP) - Version 1.0

Reference: Chouvarine P, Saha S, Peterson DG (2008) An automated, high-throughput sequence read classification pipeline for preliminary genome characterization.  Analytical Biochemistry 373: 78-87 - see Publications

Description: The SRCP is a high-throughput, automated data analysis pipeline that is used to classify genomic shotgun reads into functional/descriptive sequence categories. It represents a powerful means of rapidly describing the sequence content of a genome and a standardized method for estimating gene/repeat enrichment or reduction afforded by Cot filtration, CBCS, and/or other reduced-representation sequencing (RRS) techniques (see Peterson 2005 for review of RRS methods).

Perl Scripts: | | | | | | | | |

DTS Scripts: BulkUploadExample_DTS.txt | Example.xsd | Top3to1row_DTS.txt

SQL Queries: PossibleRepeatsQueries.txt | ProbableRepeatQueries.txt

Algorithms: DecisionTree.txt

Local Sequence Databases (LDBs): Mitochondria | Chloroplast | Transposon | EMC | Gene Index | Centromere | rDNA | Annotated Repeat

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*This material is based upon work supported by the National Science Foundation under Grant No. DBI-0421717.  Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.